Reverse transcription of the RNA was performed with ThermoScript rt-PCR (Invitrogen). Relative gene expression was determined by qRT-PCR using SYBR Green (Roche) using two housekeeping genes (proS and gdh) as reference genes [21 (link)]. All samples were run in biological triplicates. Primers are listed in Table B in S1 File and data were analyzed with LinRegPCR [22 (link)].
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