Protocol detail

Quantitative Telomere Length Analysis

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Analysis of patient telomere length was performed on QuantStudio (Life Technologies) with iTaq Sybr (Biorad). Reference DNA was generated by the combination of five healthy controls and used to produce a standard curve to determine relative telomere/single copy gene ratio (11 (link), 12 (link)).

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Espinosa J., Herr F., Tharp G., Bosinger S., Song M., Farris AB I.I.I., George R., Cheeseman J., Stempora L., Townsend R., Durrbach A, & Kirk A.D. (2016). CD57+ CD4 T cells underlie belatacept-resistant allograft rejection. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 16(4), 1102-1112.

Publication 2016

QuantStudio iTaq Sybr

Gene Patient Telomere

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Telomere length

control variables

Reference DNA generated by the combination of five healthy controls

controls

Positive control: Reference DNA generated by the combination of five healthy controls
Negative control: Not explicitly mentioned

Annotations

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