Neuroprotective Effects of NOS Inhibitors and IL-1ra

Thirty minute before Aβ administration, NOS inhibitors 7-Nitroindazole (7-NI) (50 mg kg⁻¹, Vinci Biochem, Florence, Italy), and N6-(1-iminoethyl)-L-lysine-dihydrochloride (L-NIL) (5 mg kg⁻¹, Vinci Biochem, Florence, Italy) were dissolved in saline (1 ml kg⁻¹, veh) and administered intraperitoneally (i.p.) (Trabace et al., 2007 (link)) according to the experimental assignment. Interleukin-1 receptor antagonist (IL-1ra, Sigma-Aldrich, Milan, Italy) was applied i.c.v. at a concentration of 100 ng 5 μl⁻¹ per rat (Taepavarapruk and Song, 2010 (link)). Drugs were administered 30 min (NOS inhibitors) or 10 min (IL-1ra) before Aβ injection based upon our previous experience and following a survey of the relevant literature (Trabace et al., 2007 (link); Choi et al., 2009 (link)).