Comprehensive Blood Analysis in COVID-19 Patients

Complete blood count (leukocyte count, platelet count, hemoglobin, and hematocrit) was performed on a Sysmex XN-9000 analyzer (Sysmex Corporation, Kobe, Hyogo, Japan) using EDTA blood. PT (Dade Innovin; Siemens, Marburg, Germany), aPTT (Dade Actin FSL; Siemens), fibrinogen concentration (Clauss method, Dade Thrombin Reagent; Siemens), D-dimer concentration (Innovance; Siemens), and Anti-Xa activity (Biophen Heparin LRT; Hyphen Biomed, Neuville-Sur-Oise, France) were measured on a Sysmex CS2100i (Sysmex Corporation) in 3.2% citrated blood. For the anti-Xa measurement, COVID-19 patient samples (18 μl) were three times diluted with reference pooled plasma (36 μl). Anti-Xa activity was determined using an LMWH calibration line (aXa-LMWH; Hyphen Biomed). UFH activity was subsequently calculated with a previously determined formula: UFH anti-Xa = 1.55 ^* LMWH anti-Xa (26 (link)). Bilirubin (Bilirubin Total, third-generation; Roche Diagnostics, Basel, Switzerland) and creatinine (Enzymatic Reagent; Roche Diagnostics) were determined in serum on a COBAS® 8000 (Roche Diagnostics).

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Hulshof A.M., Brüggemann R.A., Mulder M.M., van de Berg T.W., Sels J.W., Olie R.H., Spaetgens B., Streng A.S., Verhezen P., van der Horst I.C., Ten Cate H., Spronk H.M., van Bussel B.C, & Henskens Y.M. (2021). Serial EXTEM, FIBTEM, and tPA Rotational Thromboelastometry Observations in the Maastricht Intensive Care COVID Cohort—Persistence of Hypercoagulability and Hypofibrinolysis Despite Anticoagulation. Frontiers in Cardiovascular Medicine, 8, 654174.

Publication 2021

Dade Innovin Dade Actin FSL Dade Thrombin Reagent Innovance Biophen Heparin LRT CS2100i COBAS® 8000

Actin Anti xa Aptt Bilirubin Blood Complete blood count Covid 19 Creatinine D dimer Dade Diagnostics Edta Enzymatic Fibrinogen Hematocrit Hemoglobin Heparin Innovin Leukocyte count Patient Plasma Platelet count Serum Thrombin

Corresponding Organization : Maastricht University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Leukocyte count
Platelet count
Hemoglobin
Hematocrit
Prothrombin time (PT)
Activated partial thromboplastin time (aPTT)
Fibrinogen concentration
D-dimer concentration
Anti-Xa activity
Bilirubin
Creatinine

control variables

EDTA blood used for complete blood count measurements
3.2% citrated blood used for PT, aPTT, fibrinogen, D-dimer, and Anti-Xa activity measurements
COVID-19 patient samples diluted three times with reference pooled plasma for Anti-Xa activity measurement
Anti-Xa activity determined using an LMWH calibration line
UFH activity subsequently calculated using a previously determined formula
Bilirubin and creatinine measurements performed in serum on a COBAS® 8000 analyzer

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

Annotations

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