Histology and Immunohistochemistry Analysis

For histology and immunohistochemistry analysis, sections of 6 µm were made for hematoxylin/eosin staining. Immunohistochemical staining was performed as previously described 35 (link). Primary anti-DSPP (1/200; Zen Bioscience), anti-DMP1 (1/200; Santa Cruz Biotechnology), and anti-OCN (1/200; Zen Bioscience) antibodies were used. Detection of crossreacted secondary antibodies was performed using the DAB kit (Gene Tech, China). Accordingly, quantitative analysis of the staining was conducted by measuring the average optical density using the ImageJ software. At least 3 serial sections from each sample were examined, and at least 3 samples were included in each group.

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Zhang S., Yang Y., Jia S., Chen H., Duan Y., Li X., Wang S., Wang T., Lyu Y., Chen G, & Tian W. (2020). Exosome-like vesicles derived from Hertwig's epithelial root sheath cells promote the regeneration of dentin-pulp tissue. Theranostics, 10(13), 5914-5931.

Publication 2020

anti-DSPP anti-DMP1 anti-OCN DAB kit

Antibodies Dmp1 Eosin Gene Immunohistochemistry Optical

Corresponding Organization :

Other organizations : Sichuan University, University of Electronic Science and Technology of China

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Variable analysis

independent variables

Primary anti-DSPP (1/200; Zen Bioscience)
Primary anti-DMP1 (1/200; Santa Cruz Biotechnology)
Primary anti-OCN (1/200; Zen Bioscience) antibodies

dependent variables

Quantitative analysis of the staining by measuring the average optical density using the ImageJ software

control variables

Sections of 6 µm were made for hematoxylin/eosin staining
At least 3 serial sections from each sample were examined
At least 3 samples were included in each group

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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