Micropunch samples of ST and NAc were processed and Western blots were performed as previously described (Benskey et al., 2012 (link)). Protein bands were visualized using a PICO or FEMTO Chemiluminescence substrate (ThermoFisher) kit on a LI-COR Odyssey imager. Bands from each lane of the protein of interest were normalized to the loading control GAPDH using Image Studio version 5.2.
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