For electron microscopy, purified Exo and MV from 0.1 ml of plasma were processed for scanning electron microscopy (SEM) and transmission electron microscopy (TEM) as previously described (Shively and Miller, 2009 (link); Paolino et al., 2021b (link)).
For the immunoelectron microscopy, the samples on the grids were incubated with anti-CD81 monoclonal antibody (mAb B11, Santa Cruz Biotechnology, Heidelberg, Germany), and with 10 nm gold-conjugated goat anti-mouse immunoglobulin G (IgG) serum (Sigma-Aldrich, St. Louis, MO). Finally, nanovesicles were observed with a PHILIPS EM208S transmission electron microscope (FEI-ThermoFisher) (Federici et al., 2020 (link)).
For the immunoelectron microscopy, the samples on the grids were incubated with anti-CD81 monoclonal antibody (mAb B11, Santa Cruz Biotechnology, Heidelberg, Germany), and with 10 nm gold-conjugated goat anti-mouse immunoglobulin G (IgG) serum (Sigma-Aldrich, St. Louis, MO). Finally, nanovesicles were observed with a PHILIPS EM208S transmission electron microscope (FEI-ThermoFisher) (Federici et al., 2020 (link)).
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