RNA Sequencing of Fungal Strains

Total RNA was isolated using TRIzol Reagent (Invitrogen, USA) according to the manufacturer's instructions and treated with DNase I, RNase-free (Thermo Fisher, USA). The RNA concentration was quantified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher). The RNA integrity was determined by agarose formaldehyde gel electrophoresis and using the Agilent Bioanalyzer platform 2100 (Agilent, USA). Purity and concentration were measured using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher). A total of 12 libraries (Δmus-52 and Δpac-3 strains, cultivated in high- and low-Pi concentration media, each, in three biological replicates) were sequenced on an Illumina HiSeq2000 (Illumina, USA) sequencer platform with paired-end 100-bp reads. RNA-seq data were analyzed and validated as previously described (Martins et al., 2019 (link)) and deposited at the GEO database with accession number GSE132373.