Translocation of Cu-Zn SOD–CyaA into host cells was assayed using the CyaA fusion approach. After infection of J774.A1 cells (moi 100:1) for the indicated times in 96-well plates (105 cells per well), cells were gently washed five times with PBS and lysed. Intracellular cAMP levels were determined by cAMP enzyme immunoassay kit from Sigma-Aldrich Corporation LLC as described by the manufacturer [9 (link)].
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