Microfluidic Device for CTC Isolation

The deployed microfluidic device architecture has been reported by Warkiani et al. [20 (link),24 (link),25 (link),26 (link)], and was adopted for the isolation of CTCs from the SF. A microfluidic chip was structured as two polydimethylsiloxane (PDMS) layers (Sylgard 184 from Dow Corning, Midland, MI, USA) made by mixing liquid PDMS and curing agent in a 10:1 ratio (w/w). The top layer of the device fabricated by employing the previously reported micromolding process was bonded to a flat layer of PDMS following oxygen plasma treatment for 2 min (Harrick Plasma, Ithaca, NY, USA); 1.5 mm holes were punched at the beginning and ends of the microfluidic channel and 1.5 mm tygon tubes were inserted into each inlet/outlet.

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Rzhevskiy A.S., Kapitannikova A.Y., Vasilescu S.A., Karashaeva T.A., Razavi Bazaz S., Taratkin M.S., Enikeev D.V., Lekarev V.Y., Shpot E.V., Butnaru D.V., Deyev S.M., Thiery J.P., Zvyagin A.V, & Ebrahimi Warkiani M. (2022). Isolation of Circulating Tumor Cells from Seminal Fluid of Patients with Prostate Cancer Using Inertial Microfluidics. Cancers, 14(14), 3364.

Publication 2022

Sylgard 184 oxygen plasma

Chip Device Isolation Microfluidic device Oxygen treatment Plasma Polydimethylsiloxane

Corresponding Organization : Macquarie University

Other organizations : University of Technology Sydney, Medical University of Vienna, National Research Tomsk State University, Tomsk Polytechnic University, Guangzhou Regenerative Medicine and Health Guangdong Laboratory

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Variable analysis

independent variables

Microfluidic chip structure (two PDMS layers)
Ratio of liquid PDMS to curing agent (10:1 w/w)
Oxygen plasma treatment duration (2 min)

dependent variables

Isolation of circulating tumor cells (CTCs) from the sample fluid (SF)

control variables

Sylgard 184 PDMS from Dow Corning
Harrick Plasma device (Ithaca, NY, USA)
Tygon tubes (1.5 mm diameter) for inlet/outlet

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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