Regulation of NF-κB Signaling by CHIP

pCMV/Myc-TRAF6, TRAF2, and TRAF5 was provided by Dr. Lingqiang Zhang (State Key Laboratory of Proteomics, Beijing, China). pEFNeo/HA-CHIP, pRKIM/Myc-CHIP, and pRKIM/Myc-CHIP (H260Q) were generated in our lab as described previously.^{23 (link)} I-κBα, phosphor-I-κBα (Ser32) and anti-α-tubulin (3873p) were purchased from Cell Signaling Technology (Beverly, MA). Anti-TRAF6 (H-274) and anti-p65 (C-20) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-CHIP antibody was developed in our lab.^{16 (link)} MG132 was purchased from Millipore (Billerica, MA). Cyclohexanone (CHX) was purchased from Amresco (AMRESCO Inc., OH).

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Wang T., Li S., Yi D., Zhou G.Q., Chang Z., Ma P.X., Xiao G, & Chen D. (2018). CHIP regulates bone mass by targeting multiple TRAF family members in bone marrow stromal cells. Bone Research, 6, 10.

Publication 2018

phosphor-I-κBα (Ser32) anti-α-tubulin (3873p) Anti-TRAF6 (H-274) anti-p65 (C-20) MG132 Cyclohexanone (CHX)

Anti antibody Antibodies Chip Cyclohexanone I κbα Mg132 Phosphor Traf2 Traf5 Traf6 α tubulin

Corresponding Organization :

Other organizations : Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Rush University Medical Center, Shenzhen University, Tsinghua University, University of Michigan–Ann Arbor

Top 5 similar protocols

Variable analysis

independent variables

PCMV/Myc-TRAF6
TRAF2
TRAF5
PEFNeo/HA-CHIP
PRKIM/Myc-CHIP
PRKIM/Myc-CHIP (H260Q)
MG132
Cyclohexanone (CHX)

dependent variables

I-κBα
Phosphor-I-κBα (Ser32)

control variables

Anti-α-tubulin (3873p)

positive controls

None specified

negative controls

None specified

Annotations

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