Three TNBC cell lines (MDA-MB-231, Hs578T, and MDA-MB-157) were purchased from the American Type Cell Collection (Manassas, VA), and Mx-1 cells were purchased from CLS Cell Lines Service (Eppelheim, Germany) in 2016, and passaged in our laboratory for less than 6 months after receipt or resuscitation. All cell lines were tested monthly for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza Walkersville, Inc, Walkersville, MD) and were cultured for no more than 20 passages from the validated stocks. All cell lines were cultured in RPMI with 10% FBS at 37.0° C and 5% CO2. Eribulin-resistant MDA-MB-231 and Hs578T cells were previously established in our laboratory [23 (link)].
Eribulin was purchased from Eisai Co., Ltd. (Tokyo, Japan). Paclitaxel for in vitro use was purchased from Sigma-Aldrich (Saint Louis, MO) and for in vivo use was purchased from Nippon Kayaku Co., Ltd. (Tokyo, Japan). Recombinant human TGF-β1 (TGF-β) was purchased from R&D Systems (Minneapolis, MN). TGF-β receptor I kinase inhibitor (LY2157299) was purchased from Sellek Chemicals (Houston, TX).
Eribulin was purchased from Eisai Co., Ltd. (Tokyo, Japan). Paclitaxel for in vitro use was purchased from Sigma-Aldrich (Saint Louis, MO) and for in vivo use was purchased from Nippon Kayaku Co., Ltd. (Tokyo, Japan). Recombinant human TGF-β1 (TGF-β) was purchased from R&D Systems (Minneapolis, MN). TGF-β receptor I kinase inhibitor (LY2157299) was purchased from Sellek Chemicals (Houston, TX).
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