Integrin β1 Expression in Laryngeal Cancer

From 2010 to 2016, 60 laryngeal cancer tissues and 25 noncancerous laryngeal tissues were obtained from patients who underwent surgery at Taihe hospital, Hubei University of Medicine. The tissues were incubated with integrin β1 antibody (1:300; Abcam, Cambridge, MA, USA) at 4 °C overnight. After washing in PBS, the tissues were incubated with HRP-labeled secondary antibody (Beyotime, Jiangsu, China) for 30 min at room temperature. Finally, the specific immunostaining was visualized using an ultrasensitive streptavidin-peroxidase system (Maxim Biotech, Fuzhou, China) [15 (link)]. The intensity of integrin β1 staining in individual cases was evaluated by two independent scorers in a blinded fashion. A score > 4 was defined as high expression, and a score ≤ 4 was regarded as low expression [16 (link)].