Left ventricular function in mice was assessed using echocardiography (Philips TIS 0.8; Koninklijke Philips N.V.) and an RMV 707B transducer (frequency, 30 MHz; Siemens AG). The mice were anesthetized with isoflurane (dose for both induction and maintenance, 2%) before echocardiography. Images were obtained by identifying the interventricular septum and the left ventricular posterior wall. The left ventricular fractional shortening (LVFS; %) and left ventricular ejection fraction (LVEF; %) were automatically calculated by echocardiography. Each parameter was evaluated by calculating the average of four cardiac cycles. All mice in the present study were sacrificed by cervical dislocation at the end of this experiment.
Protocol detail
Echocardiographic Assessment of Cardiac Function in Mice
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Cardiac
Cervical
Dislocation
Echocardiography
Interventricular septum
Isoflurane
Left ventricular
Left ventricular function
Lvfs
Mice
Transducer
Ventricular ejection fraction
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Variable analysis
independent variables
- Anesthesia (isoflurane at 2% for both induction and maintenance)
- Left ventricular fractional shortening (LVFS)
- Left ventricular ejection fraction (LVEF)
- Mouse model (unspecified)
- Echocardiography equipment (Philips TIS 0.8, RMV 707B transducer at 30 MHz)
- Number of cardiac cycles evaluated (4)
- Positive control: None specified
- Negative control: None specified
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