Protocol detail

Multi-Epitope Peptide Vaccine Synthesis

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The multi-epitope peptide vaccine HisDTC was previously designed, as detailed in [23 (link)]. The peptides were synthesized by GenScript Biotech (Leiden, The Netherlands) with purity ≥ 95% and stored at −20 °C until encapsulation.
TLRL-2 (Pam3CSK4) and TLRL-3 (Poly(I:C) HMW (VacciGrade™, InvivoGen, Toulouse, France) were used as adjuvants when needed.

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Hurtado-Morillas C., Martínez-Rodrigo A., Orden J.A., de Urbina-Fuentes L., Mas A, & Domínguez-Bernal G. (2024). Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity. Animals : an Open Access Journal from MDPI, 14(4), 605.

Publication 2024

VacciGrade™,

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Variable analysis

independent variables

HisDTC multi-epitope peptide vaccine

dependent variables

Immune response

control variables

TLRL-2 (Pam3CSK4) and TLRL-3 (Poly(I:C) HMW (VacciGrade™)) used as adjuvants

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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