Quantifying Chikungunya Virus Infection

As previously described [87 (link)], Vero cells were seeded in 96-well plates and inoculated with 10-fold serial dilutions of samples for 2 h at 37°C. Cells were overlayed with 0.5% methylcellulose in MEM-alpha plus 10% FBS and incubated for 18 h at 37°C. Following fixation with 1% paraformaldehyde (PFA), CHIKV-infected cells were detected using CHK-11 monoclonal antibody [88 (link)] at 500 ng/mL, and foci were visualized with TrueBlue Peroxidase substrate (SeraCare 5510–0030) and counted using a CTL Biospot analyzer and Biospot software (Cellular Technology).

Free full text: Click here

Ware B.C., Parks M.G., da Silva M.O, & Morrison T.E. (2024). Chikungunya virus infection disrupts MHC-I antigen presentation via nonstructural protein 2. PLOS Pathogens, 20(3), e1011794.

Publication 2024

TrueBlue Peroxidase substrate CTL Biospot analyzer Biospot software

Corresponding Organization : Universidade Federal do Rio de Janeiro

Top 5 similar protocols

Variable analysis

independent variables

10-fold serial dilutions of samples

dependent variables

CHIKV-infected cells detected using CHK-11 monoclonal antibody
Foci counted using a CTL Biospot analyzer and Biospot software

control variables

Vero cells seeded in 96-well plates
Cells overlayed with 0.5% methylcellulose in MEM-alpha plus 10% FBS
Incubation duration of 18 h at 37°C
Fixation with 1% paraformaldehyde (PFA)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!