Plasma Sample Preparation for Metabolic Profiling

At the end of the experiment, all mice were anaesthetized with sodium pentobarbital (50 mg/kg) intraperitoneally. The eyes were removed to collect the blood samples in 5 mL Vacutainer tubes containing the chelating agent ethylene diamine tetraacetic acid (EDTA). The samples were centrifuged at 4 ℃ for 15 min, and plasma samples were collected and stored at − 80 °C. Serum random blood glucose and lipid profiles such as Total cholesterol (TC), total triglycerides (TG), High-density lipoproteins (HDL) and Low-density lipoproteins (LDL) were measured with an Abbott Architect c16000 instrument (The First Affiliated Hospital of Shantou University Medical College).

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Chen L., Wang X.J., Chen J.X., Yang J.C., Ling L.i.n., Cai X.B, & Chen Y.S. (2023). Caffeine ameliorates the metabolic syndrome in diet-induced obese mice through regulating the gut microbiota and serum metabolism. Diabetology & Metabolic Syndrome, 15, 37.

Publication 2023

c16000

Blood Blood glucose Chelating agent Cholesterol Ethylene diamine tetraacetic acid Eyes High density lipoproteins Lipid Low density lipoproteins Mice Plasma Serum Sodium pentobarbital Triglycerides

Corresponding Organization :

Other organizations : Shantou University, First Affiliated Hospital of Shantou University Medical College

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Variable analysis

independent variables

Sodium pentobarbital (50 mg/kg) administered intraperitoneally

dependent variables

Serum random blood glucose
Total cholesterol (TC)
Total triglycerides (TG)
High-density lipoproteins (HDL)
Low-density lipoproteins (LDL)

control variables

Blood collection in 5 mL Vacutainer tubes containing the chelating agent ethylene diamine tetraacetic acid (EDTA)
Centrifugation of samples at 4 ℃ for 15 min
Plasma samples collected and stored at − 80 °C
Measurement of serum parameters using an Abbott Architect c16000 instrument

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