Sequencing of Klebsiella pneumoniae from Pig Isolates

DNA extraction from pure cultures of the Norwegian KpSC pig isolates was performed by using a MagNA Pure 96 instrument (Roche) with the DNA/Viral NA SV 2.0 kit and the pathogen universal 200 4.0 protocol. Sequencing library preparation was performed by using the Illumina Nextera DNA Flex library prep kit (Illumina), followed by sequencing on an Illumina MiSeq instrument, producing paired-end reads with a length of 300 bp.
The genomes of the KpSC originating from pig isolates from Thailand were available from a previous study [16 (link)]. A total of 87 genomes were included.

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Kaspersen H., Franklin-Alming F.V., Hetland M.A., Bernhoff E., Löhr I.H., Jiwakanon J., Urdahl A.M., Leangapichart T, & Sunde M. (2023). Highly conserved composite transposon harbouring aerobactin iuc3 in Klebsiella pneumoniae from pigs. Microbial Genomics, 9(2), mgen000960.

Publication 2023

MagNA Pure 96 Nextera DNA Flex library prep kit

Dna library Dna viral Genomes Pathogen

Corresponding Organization :

Other organizations : Norwegian Veterinary Institute, University of Bergen, Stavanger University Hospital, Khon Kaen University

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Variable analysis

independent variables

DNA extraction method (MagNA Pure 96 instrument with DNA/Viral NA SV 2.0 kit and pathogen universal 200 4.0 protocol)
Sequencing library preparation method (Illumina Nextera DNA Flex library prep kit)
Sequencing platform (Illumina MiSeq instrument)

dependent variables

Paired-end reads with a length of 300 bp

control variables

Pure cultures of the Norwegian KpSC pig isolates
Genomes of the KpSC originating from pig isolates from Thailand (from a previous study)

controls

Positive control: Genomes of the KpSC originating from pig isolates from Thailand (from a previous study)
Negative control: Not specified

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