Protocol detail

Cloning and Purification of VDR-LBD

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The VDR-LBDmt DNA was kindly provided by D. Moras[6 (link)] and cloned into the pMAL-c2X vector (New England Biolabs). A detailed expression and purification protocol for VDR-LBD was reported previously.[7 (link)]

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Teske K., Nandhikonda P., Bogart J.W., Feleke B., Sidhu P., Yuan N., Preston J., Goy R., Han L., Silvaggi N.R., Singh R.K., Bikle D.D., Cook J.M, & Arnold L.A. (2014). IDENTIFICATION OF VDR ANTAGONISTS AMONG NUCLEAR RECEPTOR LIGANDS USING VIRTUAL SCREENING. Nuclear receptor research, 1, 101076.

Publication 2014

pMAL-c2X vector

Vector

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Variable analysis

independent variables

Use of the VDR-LBDmt DNA provided by D. Moras
Cloning of the VDR-LBDmt DNA into the pMAL-c2X vector

dependent variables

Outcomes of the expression and purification protocol for VDR-LBD

control variables

No control variables explicitly mentioned

controls

No positive or negative controls explicitly mentioned

Annotations

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