Genome sizes were measured for 134 individuals. Due to a lack of material, we could not measure the genome sizes of C. neapolitanus and C. siculus. Genome sizes for C. bertiscensis were partially taken from Raca et al. [16 (link)].
Genome size was determined using propidium iodide (PI) as a stain in flow cytometry with a Cyflow Space (Sysmex Partec) flow cytometer, following essentially the procedure described in Jakob et al. [6 (link)]. We mainly used rye (Secale cereale; 16.01 pg/2C) or pea (Pisum sativum; 9.09 pg/2C) as internal size standards and the buffer CyStain PI Absolute P (Sysmex Partec). Genome size measurements aimed at identifying diploids and polyploids. To link the genome sizes with the molecular data, we used silica-gel-dried leaves from the same individual used for DNA extraction whenever possible. Initial tests showed that fresh and silica-gel-dried materials arrived at the same genome size estimations in Crocus. However, the quality of data obtained is slightly lower for dried leaves. A detailed overview of the material measured and standards used is given inSupplementary Table S3 .
Genome size was determined using propidium iodide (PI) as a stain in flow cytometry with a Cyflow Space (Sysmex Partec) flow cytometer, following essentially the procedure described in Jakob et al. [6 (link)]. We mainly used rye (Secale cereale; 16.01 pg/2C) or pea (Pisum sativum; 9.09 pg/2C) as internal size standards and the buffer CyStain PI Absolute P (Sysmex Partec). Genome size measurements aimed at identifying diploids and polyploids. To link the genome sizes with the molecular data, we used silica-gel-dried leaves from the same individual used for DNA extraction whenever possible. Initial tests showed that fresh and silica-gel-dried materials arrived at the same genome size estimations in Crocus. However, the quality of data obtained is slightly lower for dried leaves. A detailed overview of the material measured and standards used is given in
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