Bone mineral density distributions (BMDD) were assessed using quantitative backscattered scanning electron microscopy (bSEM). Embedded and polished samples used for FTIRM were carbon coated (Crestington) and imaged at 25 keV, a working distance of 19 mm, and a magnification of 150× (Zeiss Sigma HDVP) using techniques previously described elsewhere [16 (link)].