Establishment and Characterization of Oral Cancer Cell Lines

ORL-48, ORL-204 and ORL-188 oral cancer cell lines isolated respectively from gingiva (gum), buccal mucosa (lining of the cheeks and back of the lips) and tongue were obtained from Cancer Research Malaysia (Subang Jaya, Selangor). These cells were established from surgically resected specimens obtained from untreated primary human oral squamous cell carcinomata of the oral cavity as in vitro models to study a disease prevalent in Asia. Their growth characteristics, epithelial origin and molecular alterations were previously characterized (24 (link), 25 (link)). Genetic information and clinical data associated with these ORL cell lines are available at https://genipac.cancerresearch.my/ (26 (link)). ORL-48, ORL-204 and ORL-188 cells were cultured and maintained in DMEM/F-12 medium (Nacalai Tesque Inc., Kyoto, Japan) supplemented with 10% foetal bovine serum (Nacalai Tesque Inc.) and 0.1% penicillin-streptomycin (Sigma-Aldrich, Merck) at 37 °C and 5% CO₂.

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Yap H.Y., Kong B.H., Yap C.S., Ong K.C., Zain R.B., Tan S.H., Zaini Z.M., Ng S.T., Tan C.S, & Fung S.Y. (2022). Immunomodulatory Effect and an Intervention of TNF Signalling Leading to Apoptotic and Cell Cycle Arrest on ORL-204 Oral Cancer Cells by Tiger Milk Mushroom, Lignosus rhinocerus. Food Technology and Biotechnology, 60(1), 80-88.

Publication 2022

DMEM/F-12 medium foetal bovine serum penicillin-streptomycin

Buccal mucosa Cancer Cell lines Cells Cheeks Foetal bovine serum Human Information Lips Oral cancer Penicillin Squamous cell carcinomata of the oral cavity Streptomycin Surgically Tongue

Corresponding Organization :

Other organizations : Mahsa University, University of Malaya

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Variable analysis

independent variables

ORL-48 oral cancer cell line
ORL-204 oral cancer cell line
ORL-188 oral cancer cell line

dependent variables

Growth characteristics
Epithelial origin
Molecular alterations

control variables

Culture medium (DMEM/F-12 supplemented with 10% foetal bovine serum and 0.1% penicillin-streptomycin)
Temperature (37 °C)
Carbon dioxide concentration (5% CO2)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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