6–12-wk-old female C57BL/6J mice were injected i.p., unless otherwise noted, with 0.5 mg whole ovalbumin (Sigma-Aldrich) with or without varying amounts of TLR agonists and/or 50 μg of 1C10 or FGK45 (anti-CD40). Where peptide injections are noted, mice were injected with anti-CD40 i.p. and then 4–6 h later injected i.v. with 100 mg SIINFEKL peptide and a given TLR agonist. Anti-CD40 was used at 50 μg per injection. Mice were immunized with a single injection i.p. and killed 6 d later unless otherwise noted.