Digital color-coded barcode fluorescence reporter (nCounter Elements TagSet-12) was purchased from NanoString Technologies, Inc. Synthetic nucleic acid probe A (84-mer) and capture probe modified with photocleavable spacer/biotin (58-mer) were obtained from Integrated DNA Technologies, Inc. (IDT). They were designed to complement part of the Zaire Ebola Virus (AY354458.1:12638) and the barcode fluorescence reporter (for sequences see Table S1). Purified Zaire Ebola Virus RNA (AY354458.1) was extracted in the Biosafety Level 4 Laboratory at Texas Biomedical Research Institute using Trizol LS reagents (Thermo Fisher Scientific) as reported previously.28 (link),35 (link),36 (link) Purified Ebola RNA was spiked in mouse whole blood samples containing sodium heparin to avoid coagulation (Biochemed Services) for on-chip microfluidic experiments. MyOne streptavidin T1 beads (10 mg/mL) were purchased from Thermo Fisher Scientific and washed 4 times with RNase-free water (Takara Bio Inc.) for on-chip experiments.