SDS-PAGE was performed as described.15 (link),29 (link) The following antibodies were used: rabbit anti-β1 (1:100; Abgent), mouse anti-fyn (1:1,000; BioLegend), mouse anti-CD44 (1:1,000; AbD Serotec); rabbit anti-E-cadherin (1:1,000; Cell Signaling Technology); mouse anti-β-actin (1:30,000; Proteintech); and mouse anti-α-tubulin (1:10,000; Sigma). Signals were quantified using ImageJ software. α-tubulin was used as loading control.
Free full text: Click here