Adoptive Transfer of B Cells

B cells from MD4 or MD4/ML5 mice treated with anti-CD79b mAb or control IgG were enriched by depletion of CD43+ cells with magnetic beads (MACS anti-mouse CD43; Miltenyi Biotec). A total of 10⁶ B cells in 200 ml PBS was adoptively transferred by i.v. injection. One hour later the recipient mice were immunized i.p. with HEL-conjugated SRBCs as described previously (29 (link)).

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Hardy I.R., Anceriz N., Rousseau F., Seefeldt M.B., Hatterer E., Irla M., Buatois V., Chatel L.E., Getahun A., Fletcher A., Cons L., Pontini G., Hertzberg N.A., Magistrelli G., Malinge P., Smith M.J., Reith W., Kosco-Vilbois M.H., Ferlin W.G, & Cambier J.C. (2014). Anti-CD79 Antibody Induces B cell Anergy That Protects Against Autoimmunity. Journal of immunology (Baltimore, Md. : 1950), 192(4), 1641-1650.

Publication 2014

MACS anti-mouse CD43

B cells Cd43 Cd79b Cells Mice

Corresponding Organization :

Other organizations : National Jewish Health, University of Colorado Denver, University of Geneva

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Variable analysis

independent variables

Treatment with anti-CD79b mAb
Treatment with control IgG

dependent variables

Adoptive transfer of B cells
Immunization with HEL-conjugated SRBCs

control variables

B cells from MD4 or MD4/ML5 mice
Depletion of CD43+ cells with magnetic beads
Total of 10^6 B cells in 200 ml PBS for adoptive transfer
Time between adoptive transfer and immunization (1 hour)

controls

Positive control: Immunization with HEL-conjugated SRBCs
Negative control: Not explicitly mentioned

Annotations

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