HT1080 cells were transfected by the same procedure previously outlined in [29 (link)]. shRNA constructs targeting the MMP 1, MMP 9, MMP 7, MMP 11 genes were purchased from Sigma Aldrich. PCR studies were performed after lentiviral mediated transduction and only shRNAs showing more than 85% knockdown were used for subsequent studies (Supplementary Figure 2H–2K ). Genomic sequences for the knockdowns are listed in Table 1 .
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