Immunofluorescence Analysis of Tight Junction Proteins

The rat anti-occludin monoclonal antibody (mAb), rabbit anti-occludin polyclonal antibody (pAb) [17 (link)], rat anti-angulin-1/LSR mAb, rabbit anti-angulin-2/ILDR1 pAb [9 (link)], and rat anti-tricellulin mAb (24–69) [7 (link)] were characterized as described previously. The rabbit anti-cleaved-Caspase-3 (Asp175) pAb (# 9661) was purchased from Cell Signaling Technology (Danvers, MA, USA). Alexa Fluor 546-phalloidin (#A22283), Alexa Fluor 488 goat anti-rabbit IgG pAb (#A11008) and Alexa Fluor 546 goat anti-rat IgG pAb (#A11081) were purchased from Life Technologies (Waltham, MA, USA).

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Higashi T., Katsuno T., Kitajiri S.I, & Furuse M. (2015). Deficiency of Angulin-2/ILDR1, a Tricellular Tight Junction-Associated Membrane Protein, Causes Deafness with Cochlear Hair Cell Degeneration in Mice. PLoS ONE, 10(3), e0120674.

Publication 2015

Alexa Fluor 546-phalloidin

Alexa fluor 488 Alexa fluor 546 Anti antibody Anti igg Antibody Caspase 3 Goat Monoclonal antibody Occludin Phalloidin Rabbit Tricellulin

Corresponding Organization : The Graduate University for Advanced Studies, SOKENDAI

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Variable analysis

independent variables

Rat anti-occludin monoclonal antibody (mAb)
Rabbit anti-occludin polyclonal antibody (pAb)
Rat anti-angulin-1/LSR mAb
Rabbit anti-angulin-2/ILDR1 pAb
Rat anti-tricellulin mAb (24–69)
Rabbit anti-cleaved-Caspase-3 (Asp175) pAb

dependent variables

Not explicitly mentioned

control variables

Alexa Fluor 546-phalloidin
Alexa Fluor 488 goat anti-rabbit IgG pAb
Alexa Fluor 546 goat anti-rat IgG pAb

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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