Quantitative Analysis of Hexoses and Pentoses

A UV—vis opectrophotometer (756 PC, Shanghai Hongji Instruments Co., Ltd., Shanghai, China) was used for absorbance reading values. The Anthrone/H₂SO₄ method [48 ] was used to determine hexoses and the orcinol/HCl method [49 ] was used to determine pentoses. The details of the determination method were carried out according to Xu et al [50 (link)]. Anthrone and orcinol were purchased from Sigma-Aldrich Co., LLC and ferric chloride was obtained from Sinopharm Chemical Reagent Co., Ltd. D-glucose and D-xylose purchased from Sigma-Aldrich Co., LLC were used as standards for creating standard curves, with R² higher than 0.9990, of hexoses and pentoses.