Quantifying post-MI immune cells

The mice were euthanized at 4 weeks after MI. Their blood, spleens, bone marrow and infarcted hearts were collected and made into single-cell suspensions for flow cytometry analysis, as previously described10 (link). The cells were stained with a mixture of antibodies (anti-CD11b-APC and anti-Gr-1-PerCP-Cy5.5 BD Biosciences). The data were acquired using an LSRII flow cytometer (BD Biosciences) and were analyzed with FlowJo7 software (Tree Star, Inc.).

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Chen J., Hong T., Ding S., Deng L., Abudupataer M., Zhang W., Tong M., Jia J., Gong H., Zou Y., Wang T.C., Ge J, & Yang X. (2017). Aggravated myocardial infarction-induced cardiac remodeling and heart failure in histamine-deficient mice. Scientific Reports, 7, 44007.

Publication 2017

anti-CD11b-APC anti-Gr-1-PerCP-Cy5.5 LSRII flow cytometer FlowJo7 software

Antibodies Blood Bone marrow Cd11b Cells Cy5 5 Flow cytometry Hearts Mice Tree

Corresponding Organization :

Other organizations : The First Affiliated Hospital, Sun Yat-sen University, Zhongshan Hospital, Fudan University, Sun Yat-sen University, Shanghai Jiao Tong University, Tongren Hospital, Columbia University

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Variable analysis

independent variables

Time after MI (4 weeks)

dependent variables

Cell populations in blood, spleen, bone marrow, and infarcted heart

control variables

Staining with antibodies (anti-CD11b-APC and anti-Gr-1-PerCP-Cy5.5)

controls

Positive control: Not specified
Negative control: Not specified

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