Synthesis and Purification of RNA Oligonucleotides

Synthetic oligoribonucleotides were obtained from GE Healthcare Dharmacon (U30, Het30) or TriLink Biotechnologies, Inc. (ppp-Het30), dT30 was obtained from IDT, Inc. ss-dsRNAs were prepared by in vitro transcription as previously described^{22 (link)}. For RNAs containing 4-thiouridine, UTP was replaced with 4-Thio-UTP (TriLink Biotechnologies, Inc.) in the transcription reactions. All RNAs were purified by denaturing PAGE followed by electroelution in an Elutrap device (Schleicher and Schuell).

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Mayo C.B, & Cole J.L. (2017). Interaction of PKR with single-stranded RNA. Scientific Reports, 7, 3335.

Publication 2017

4-Thio-UTP

4 thiouridine Device Dsrnas Oligoribonucleotides Rnas Transcription

Corresponding Organization :

Other organizations : University of Connecticut

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Variable analysis

independent variables

Type of oligoribonucleotides obtained (U30, Het30, ppp-Het30, dT30)
Replacement of UTP with 4-Thio-UTP for RNAs containing 4-thiouridine

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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