SARS-CoV-2 Infection Detection via RT-qPCR

Quantitative real-time PCR (RT qPCR) was performed as described previously (20 (link)) at the time of sample collection for flow cytometry experiments. This procedure was essential to ensure that no SARS-CoV-2 infection or reinfection occurred in the HC and recovered volunteers. The RNA isolated from nasopharyngeal or oropharyngeal swabs was extracted (QIAprep& Viral RNA UM Kit, QIAGEN, USA) and amplified by one-step RT qPCR (SARS-CoV-2 N1+N2 Assay Kits, QIAGEN, USA).

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de Sousa Palmeira P.H., Peixoto R.F., Csordas B.G., de Medeiros I.A., de Azevedo F.D., Veras R.C., Janebro D.I., Amaral I.P, & Keesen T.S. (2023). Differential regulatory T cell signature after recovery from mild COVID-19. Frontiers in Immunology, 14, 1078922.

Publication 2023

QIAprep& Viral RNA UM Kit SARS-CoV-2 N1+N2 Assay Kits

Assay Flow cytometry Nasopharyngeal Oropharyngeal Real time pcr Reinfection Sample collection Sars cov 2 Sars cov 2 infection Viral rna Volunteers

Corresponding Organization : Universidade Federal da Paraíba

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

SARS-CoV-2 infection or reinfection status in the HC and recovered volunteers

control variables

Procedures were followed to ensure that no SARS-CoV-2 infection or reinfection occurred in the HC and recovered volunteers

controls

Positive control: Not specified
Negative control: Not specified

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