Histopathological Investigation of CNS Inflammation

For histopathological investigation, the skull was removed and the head was fixed in 4% neutral‐buffered formalin for at least 1 week followed by decalcification for 3 days in Formical 2000 (Decal, Tallman, NY). From all heads, eight coronal head sections were obtained, embedded in paraffin wax and cut at 3 or 5 µm thick slices, respectively, for further histological and immunohistochemical evaluation [28 (link)]. The slices were mounted on Super‐Frost‐Plus‐Slides (Carl Roth GmbH, Karlsruhe, Germany) and stained with hematoxylin–eosin for detailed neuropathological analysis of CNS inflammation.