Confocal immunofluorescence microscopy was performed on LSM510 (Carl-Zeiss) as described [60 ,61 (link)]. HeLa cells were transfected with an S-expressing plasmid and pDsRed-ER (Clontech) for 36 h. Cells were then fixed and stained with anti-V5 antibody. Nuclei were counter-stained with 4', 6-diamidino-2-phenylindole (DAPI) before mounting.
Free full text: Click here