Myc-SNAP was amplified from pSNAPf (NEB) as a 5’XbaI-3’SpeI PCR fragment and cloned into the XbaI-SpeI digested ManII-Halo (Bottanelli et al., 2016 (link)).
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Protocol detail
Myc-SNAP Cloning into ManII-Halo
Corresponding Organization : Yale University
Other organizations : University of Cambridge, Wellcome/Cancer Research UK Gurdon Institute, Wellcome Trust
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Variable analysis
independent variables
- Myc-SNAP amplified from pSNAP
f (NEB) as a 5'XbaI-3'SpeI PCR fragment
- Not explicitly mentioned
- ManII-Halo (Bottanelli et al., 2016 (link))
- Positive control: Not specified
- Negative control: Not specified
Annotations
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