Western blotting was carried out as described previously [27 (link)]. In brief, an equal volume of cellular lysate was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the proteins were transferred to a PVDF membrane. The membranes were probed with antibodies specific to DENV NS2B (1:5000; Abcam, Cambridge, MA, USA), anti-GAPDH (1:10,000; GeneTex, Irvine, CA, USA), anti-COX-2 antibody (1:1000; Cayman Chemical, Ann Arbor, MI, USA), anti-MAPK (phosphorylated and unphosphorylated forms of ERK1/2, p38, and JNK), and anti-C-Myc antibody (1:1000; GeneTex).
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