Characterization of E. coli and K. pneumoniae

Escherichia coli strains MG1655, MG1655(ΔsbmA) (Ghosal et al., 2013 (link)), AS19 (Sekiguchi and Iida, 1967 (link)), ATCC25922(ΔrfaG) (Ebbensgaard et al., 2018 (link)), and Klebsiella pneumoniae ATCC 13883 were used throughout the study and cultured in non-cation adjusted Muller-Hinton Broth (MHB) (Sigma-Aldrich, cat.no. 70192) at 37°C.
Peptide nucleic acids (Table 1) were dissolved in water and the concentration determined using a NanoDrop spectrophotometer at 260 nm. Low binding plastics (Axygen, Corning) were used throughout.

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Goltermann L., Yavari N., Zhang M., Ghosal A, & Nielsen P.E. (2019). PNA Length Restriction of Antibacterial Activity of Peptide-PNA Conjugates in Escherichia coli Through Effects of the Inner Membrane. Frontiers in Microbiology, 10, 1032.

Publication 2019

MHB Axygen

Escherichia coli Klebsiella pneumoniae Peptide nucleic acids Strains

Corresponding Organization : University of Copenhagen

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Variable analysis

independent variables

Escherichia coli strains MG1655, MG1655(ΔsbmA), AS19, ATCC25922(ΔrfaG)
Klebsiella pneumoniae ATCC 13883

dependent variables

Not explicitly mentioned

control variables

Cultured in non-cation adjusted Muller-Hinton Broth (MHB) (Sigma-Aldrich, cat.no. 70192) at 37°C
Low binding plastics (Axygen, Corning) used throughout

controls

No positive or negative controls were explicitly mentioned

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