Cell Culture Protocols for Various Cell Types

SW1353 and OUMS-27 human CS cells, C28/I2 chondrocytes (obtained from Dr. M. Goldring, Hospital for Special Surgery, New York, NY, USA), and 4T1.2 mouse mammary tumor cells (obtained from Dr. R. Anderson at Peter MacCallum Cancer Institute, Melbourne, Australia) were cultured in DMEM [26 (link),27 (link),28 (link),29 (link)] RAW264.7 pre-osteoclast cells (ATCC, Manassas, VA, USA) were grown in αMEM [30 (link)]. Human MSCs (Lonza, Basel, Switzerland) were grown in MSC Basal Medium (Lonza). MH7A synovial cells (Riken Cell Bank, Tsukuba, Japan) were cultured in RPMI1640 [31 (link)]. The culture media were supplemented with 10% FBS (fetal bovine serum) and antibiotics (penicillin and streptomycin), and cells were maintained at 37 °C and 5% CO₂.

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Sun X., Li K.X., Figueiredo M.L., Lin C.C., Li B.Y, & Yokota H. (2022). Generation of the Chondroprotective Proteomes by Activating PI3K and TNFα Signaling. Cancers, 14(13), 3039.

Publication 2022

RAW264.7 pre-osteoclast cells Human MSCs MSC Basal Medium MH7A synovial cells

Antibiotics Cancer Cells Chondrocytes Culture media Fetal bovine serum Human Mammary tumor Mouse Osteoclast Penicillin Raw264 7 cells Streptomycin Surgery Synovial cells

Corresponding Organization : Indiana University Health

Other organizations : Purdue University West Lafayette

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Variable analysis

independent variables

Cell types: SW1353 and OUMS-27 human CS cells, C28/I2 chondrocytes, 4T1.2 mouse mammary tumor cells, RAW264.7 pre-osteoclast cells, human MSCs, MH7A synovial cells

dependent variables

Not explicitly mentioned

control variables

Culture media: DMEM, αMEM, MSC Basal Medium, RPMI1640
Supplements: 10% FBS, antibiotics (penicillin and streptomycin)
Incubation conditions: 37 °C, 5% CO2

controls

No positive or negative controls were explicitly mentioned

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