RNAs for GCaMP6s and PKCδ-GFP were synthesised from pCS2-based plasmids containing the respective coding sequences (Sivak et al., 2005 (link); Chen et al., 2017 (link)). These were linearised with NotI (NEB), and RNA in vitro transcribed with mMESSAGE mMACHINE SP6 Transcription Kit (Ambion). RNA for Tol2 was generated from the pT3Ts-Tol2 plasmid, linearised with SmaI (NEB), and transcribed with the mMESSAGE mMACHINE T3 Transcription Kit (Ambion). RNA for injection was purified by lithium chloride precipitation.
Ma J., Scott C.A., Ho Y.N., Mahabaleshwar H., Marsay K.S., Zhang C., Teow C.K., Ng S.S., Zhang W., Tergaonkar V., Partridge L.J., Roy S., Amaya E, & Carney T.J. (2021). Matriptase activation of Gq drives epithelial disruption and inflammation via RSK and DUOX. eLife, 10, e66596.
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
