FGFR2 Protein Level Quantification

FGFR2 protein level was examined using immunohistochemistry and quantified in digitalized slides as described previously [20 (link)]. Immunohistochemical staining (IHC) was conducted on 5‐μm paraffin tumour sections using a mouse monoclonal anti‐FGFR2 antibody (H00002263‐M01; clone 1G3, Abnova, Taipei City, Taiwan). All slides were digitalized (Pannoramic 1000 Scanner, 3DHistech, Sysmex, Kobe, Japan) for quantification in 0–300 H‐score scale. Cases from 1st tercile of H‐score were regarded as FGFR2low and cases from 2nd and 3rd terciles were classified as FGFR2high.

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Mieczkowski K., Kitowska K., Braun M., Galikowska‐Bogut B., Gorska‐Arcisz M., Piasecka D., Stawiski K., Zaczek A.J., Nejc D., Kordek R., Romanska H.M, & Sadej R. (2022). FGF7/FGFR2–JunB signalling counteracts the effect of progesterone in luminal breast cancer. Molecular Oncology, 16(15), 2823-2842.

Publication 2022

H00002263‐M01; clone 1G3 Pannoramic 1000 Scanner

Anti antibody Clone Fgfr2 Fgfr2 protein Immunohistochemistry Mouse Paraffin Tumour

Corresponding Organization : Medical University of Lodz

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Variable analysis

independent variables

FGFR2 protein level

dependent variables

FGFR2 protein expression level (quantified in digitalized slides)

control variables

Mouse monoclonal anti-FGFR2 antibody (H00002263-M01; clone 1G3, Abnova, Taipei City, Taiwan) used for immunohistochemical staining
Digitalization of slides using Pannoramic 1000 Scanner (3DHistech, Sysmex, Kobe, Japan)
Quantification of FGFR2 protein expression in 0-300 H-score scale

controls

No positive or negative controls were explicitly mentioned in the provided information.

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