Four PDAC cells (PANC-1, BXPC-3, ASPC-1, and CFPAC-1) are purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China). Thereinto, PANC-1 and BXPC-3 use Dulbecco’s Modified Eagle’s medium (DMEM) (Life technologies, South America) containing 10% fetal bovine serum (Life technologies, South America), and ASPC-1 use Roswell Park Memorial Institute-1640 (RPMI-1640) medium (Life technologies, South America) containing 10% fetal bovine serum (Life technologies, South America). CFPAC-1 use Iscove’s Modified Dulbecco Medium (IMDM) (Life technologies, South America) containing 10% fetal bovine serum (Life technologies, South America). Cells are cultured in 37 °C incubator with air conditions of 5% CO2/95% air, and resuscitate cells every 3 to 4 months. Preparation and transfection of HSP27-siRNA are conducted as described previously (Table S1 ) (11 (link)).
Protocol detail
PDAC Cell Cultivation and siRNA Transfection
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Corresponding Organization : Nanchang University
Other organizations : Zhongnan Hospital of Wuhan University, Wuhan University
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Variable analysis
independent variables
- HSP27-siRNA transfection
- Not explicitly mentioned
- Cell culture conditions (37 °C incubator with 5% CO2/95% air)
- Cell line (PANC-1, BXPC-3, ASPC-1, CFPAC-1)
- Cell culture media (DMEM, RPMI-1640, IMDM) with 10% fetal bovine serum
- Cell passage intervals (3-4 months)
- Positive control: None mentioned
- Negative control: None mentioned
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