Preparation and Characterization of Ginkgo biloba Extract

In ordinary saline, indomethacin (Sigma-Aldrich, St. Louis, MO, USA) was dissolved. The Arab Company for Pharmaceuticals and Medicinal Plants, Egypt, provided the standardized G. biloba leaf extract (EGb 761) as a pure powder (Batch No.: 510421, Registration No.: 888/2011). It was dissolved to a final concentration of 40 mg/mL in 0.25% carbox-ymethylcellulose (CMC; Sigma-Aldrich, St. Louis, MO, USA) in saline. EGb 761 was heated and vortexed to assure its solubility before being stored at 4 C for 24 h prior to administration. According to Chassagne et al. [81 (link)], voucher specimens (GEO20494, GEO20496, and GEO20497) are kept at the Emory University Herbarium in Atlanta, Georgia, in the United States [87 (link)].