Quantity of the PLZF gene expression (Mm01176868_
m1) in the neonate SSCs, adult SSCs, ES cells, and
ES-like cells were examined by dynamic array chips(Fluidigm). Glyceraldehyde-3-phosphate dehydrogenase
(GAPDH, Mm99999915_g1) was used as housekeepinggene for normalization. Cultured cells were selected with amicromanipulator, lysed with lysis buffer solution containing
1.3 µl TE buffer, 0.2 µl RT/Taq Superscript III (Invitrogen,
USA), 9 µl RT-PreAmp Master Mix, 5.0 µl Cells Direct2× Reaction Mix (Invitrogen, USA), and 2.5 µl 0.2× assay
pool. Using TaqMan real-time PCR on the BioMark Real-
Time quantitative PCR (qPCR) system, the amount of RNA-
targeted copies was evaluated. Samples were examined intwo technical repeats. The Ct values were analyzed by GenEx
software from the MultiD analysis (2 (link), 3 (link), 6 (link)).
m1) in the neonate SSCs, adult SSCs, ES cells, and
ES-like cells were examined by dynamic array chips(Fluidigm). Glyceraldehyde-3-phosphate dehydrogenase
(GAPDH, Mm99999915_g1) was used as housekeepinggene for normalization. Cultured cells were selected with amicromanipulator, lysed with lysis buffer solution containing
1.3 µl TE buffer, 0.2 µl RT/Taq Superscript III (Invitrogen,
USA), 9 µl RT-PreAmp Master Mix, 5.0 µl Cells Direct2× Reaction Mix (Invitrogen, USA), and 2.5 µl 0.2× assay
pool. Using TaqMan real-time PCR on the BioMark Real-
Time quantitative PCR (qPCR) system, the amount of RNA-
targeted copies was evaluated. Samples were examined intwo technical repeats. The Ct values were analyzed by GenEx
software from the MultiD analysis (2 (link), 3 (link), 6 (link)).
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