Mice underwent PAB surgery to induce RVF. PAB gradient was confirmed by echocardiography. At the time of surgery, animals were randomly assigned to receive locked nucleic acid (LNA)‐anti‐miR‐34a (25 mg/kg) intraperitoneally (n=9) or scramble control (n=8). Clinical and echocardiographic assessment was performed weekly, and animals were euthanized at the onset of overt heart failure. Capillary assessment and miR and protein expression studies were performed as described above in a subset of animals based on sample availability.
Protocol detail
LNA-anti-miR-34a Therapy for RVF
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Corresponding Organization :
Other organizations : Cardiovascular Institute of the South, Stanford University, University Hospital Cologne
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Variable analysis
independent variables
- Locked nucleic acid (LNA)‐anti‐miR‐34a (25 mg/kg) intraperitoneally
- Scramble control
- Clinical assessment
- Echocardiographic assessment
- Capillary assessment
- MiR expression
- Protein expression
- Mice underwent PAB surgery to induce RVF
- PAB gradient was confirmed by echocardiography
- Animals were randomly assigned to treatment groups
- Scramble control
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