Human colorectal adenocarcinoma cells, HT-29, Lovo, and HCT-116 were purchased from American Type Culture Collection (Manassas, VA, USA). Antibodies for ATF3 were purchased from Santa Cruz (Santa Cruz, CA, USA). Antibodies for GSK3β, PARP, actin, phospho-AKT (Ser473) and total AKT were purchased from Cell Signaling (Beverly, MA, USA). The trans-10, cis-12 CLA (t10, c12-CLA) was purchased from Natural Lipids (Hovdebygda, Norway). The t10, c12-CLA preparation was 94 % pure, with 2% cis-9, trans-11 isomer (c9, t11-CLA) and 3% other conjugated linoleic acid isomers. IGF-1 was purchased from BD Biosciences (Bedford, MA, USA). Human ATF3 promoter constructs were previously reported (Lee et al., 2010a (link)). All chemicals were purchased from Fisher Scientific, unless otherwise specified.
Protocol detail
Colorectal Cancer Cell Line Characterization
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Actin
Adenocarcinoma
Antibodies
Cells
Conjugated linoleic acid
Gsk3β
Ht 29
Human
Igf 1
Isomers
Lipids
Corresponding Organization :
Other organizations : University of Maryland, College Park, University of Massachusetts Amherst
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Variable analysis
independent variables
- Trans-10, cis-12 CLA (t10, c12-CLA)
- ATF3 expression
- GSK3β expression
- PARP expression
- AKT phosphorylation
- AKT total expression
- Human colorectal adenocarcinoma cell lines: HT-29, Lovo, and HCT-116
- Antibodies for ATF3, GSK3β, PARP, actin, phospho-AKT (Ser473), and total AKT
- IGF-1
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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