Single-cell assays testing for My, Ery, Meg and Ly differentiation were performed as described in 35 . Single-cell cultures specifically promoting Ery and Meg lineage differentiation were modified from a published protocol 36 (link). Bulk colony assays were performed using the Megacult™-C kit or MethoCult™ (H4034) medium following manufacturer’s instructions (Stem Cell Technologies). Further details on cell culture assays and their analysis are described in the supplemental methods.
Mende N., Bastos H.P., Santoro A., Mahbubani K.T., Ciaurro V., Calderbank E.F., Londoño M.Q., Sham K., Mantica G., Morishima T., Mitchell E., Lidonnici M.R., Meier-Abt F., Hayler D., Jardine L., Curd A., Haniffa M., Ferrari G., Takizawa H., Wilson N.K., Göttgens B., Saeb-Parsy K., Frontini M, & Laurenti E. (2022). Unique molecular and functional features of extramedullary hematopoietic stem and progenitor cell reservoirs in humans. Blood, 139(23), 3387-3401.
Other organizations :
Stem Cell Institute, Wellcome/MRC Cambridge Stem Cell Institute, University of Cambridge, Kumamoto University, Wellcome Sanger Institute, The San Raffaele Telethon Institute for Gene Therapy, Istituti di Ricovero e Cura a Carattere Scientifico, University Hospital of Zurich, University of Zurich, ETH Zurich, Newcastle University, Newcastle upon Tyne Hospitals NHS Foundation Trust, Freeman Hospital, NIHR Newcastle Biomedical Research Centre, San Raffaele University of Rome, Vita-Salute San Raffaele University, University of Exeter, National Health Service, British Heart Foundation
Single-cell assays testing for My, Ery, Meg and Ly differentiation
Single-cell cultures specifically promoting Ery and Meg lineage differentiation
dependent variables
My, Ery, Meg and Ly differentiation
control variables
Cell culture assays and their analysis described in the supplemental methods
controls
Positive control: Not specified
Negative control: Not specified
Annotations
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