Drosophila Starvation-Survival Behavior

All statistics were computed using the Prism analysis package (GraphPad v.9). Starvation-survival curves were analyzed using Kaplan–Meier log-rank tests or Gehan–Breslow–Wilcoxon test. Other data were assessed for normality before comparisons were performed. For normally distributed data, pairwise comparisons were made using two-tailed unpaired Student’s t-tests and multiple samples were compared using one-way analysis of variance (ANOVA) with post hoc multiple-comparisons tests. Other data were compared using two-tailed unpaired Mann–Whitney U-tests or one-way Kruskal–Wallis ANOVA followed by multiple-comparisons tests. Bar plots show the mean plus or minus the standard error of the mean (s.e.m.). Box plots that show the median and the first and third quartile, with whiskers indicating the full range of values. No data were excluded. Sample size was chosen on the basis of similar previously published studies of Drosophila behaviour and metabolism^{17 (link),18 (link),20 (link),55 (link)}. No sample-size calculations were performed.