Protocol detail

Generation of CD11c+ cell-specific GP96 knockout mice

Find Similar Protocols

CD11c⁺ cell–specific GP96-deficient mice (CD11cCre⁺Hsp90b1^fl/fl) and control littermates (CD11cCre^–Hsp90b1^fl/fl) were generated by crossing our Hsp90b1^fl/fl (GP96 is encoded by Hsp90b1) mice (58 (link)) with CD11c-Cre transgenic mice (68 (link)). These GP96-deficient mice were further crossed with MMVT-PyMT transgenic mice (69 (link)) to generate MMVT-PyM-CD11cCre⁺Hsp90b1^fl/fl mice. Batf3^–/– mice were purchased from The Jackson Laboratory (stock no. 013755). All mouse models in this study are on the C57BL/6 background.

Free full text: Click here

Iwanowycz S., Ngoi S., Li Y., Hill M., Koivisto C., Parrish M., Guo B., Li Z, & Liu B. (2021). Type 2 dendritic cells mediate control of cytotoxic T cell resistant tumors. JCI Insight, 6(17), e145885.

Publication 2021

Batf3–/– mice

Cell Hsp90b1 Mouse Transgenic mice

Top 5 similar protocols

Variable analysis

independent variables

Generation of CD11c⁺ cell–specific GP96-deficient mice (CD11cCre⁺Hsp90b1^fl/fl) and control littermates (CD11cCre^–Hsp90b1^fl/fl) by crossing Hsp90b1^fl/fl (GP96 is encoded by Hsp90b1) mice with CD11c-Cre transgenic mice
Crossing GP96-deficient mice with MMVT-PyMT transgenic mice to generate MMVT-PyM-CD11cCre⁺Hsp90b1^fl/fl mice

dependent variables

Not explicitly mentioned

control variables

All mouse models are on the C57BL/6 background

positive controls

CD11cCre^–Hsp90b1^fl/fl control littermates

negative controls

Batf3^–/– mice

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!