Site-directed mutagenesis was used to create the non-glycosylation mutant GlyRα1N38Q. The human GlyRα1 cDNA in pRK5 vector (gift †P. Seeburg) was used as template. Sequence correctness was verified (Eurofins Genomics Germany GmbH, Ebersberg, Germany). For transfection with GFP, the eGFP-N1 plasmid (Takara Bio Europe, Saint-Germain-en-Laye, France) was used.
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