Generation of GlyRα1 N38Q Mutant
Corresponding Organization :
Other organizations : University of Würzburg, Universitätsklinikum Würzburg, University Hospital Heidelberg, Heidelberg University, Jena University Hospital, Istanbul University
Variable analysis
- Site-directed mutagenesis to create the non-glycosylation mutant GlyRα1^N38Q
- Sequence correctness of the GlyRα1^N38Q mutant
- Human GlyRα1 cDNA in pRK5 vector (gift from P. Seeburg) as template
- EGFP-N1 plasmid (Takara Bio Europe, Saint-Germain-en-Laye, France) for transfection with GFP
- No positive or negative controls were explicitly mentioned in the provided information.
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