Murine Infection Models for Immunology

The University of Iowa institutional animal care and use committee approved all experiments. C57BL/6 WT (JAX stock 002288), Ifngr1^−/− (JAX stock 025545), and MD4 (JAX stock 002595) mice were purchased from The Jackson Laboratory. C57BL/6 Myd88^−/− and Ticam1^−/− were generous gifts from Michelle Callegan (University of Oklahoma Health Sciences Center, Oklahoma City, OK); Tim1^−/− mice were a gift from Paul Rothman (Johns Hopkins, Baltimore, MD); and Axl^−/− bone marrow was a generous gift from Carla Rothlin (Yale University, New Haven, CT). Infections in experimental mice were initiated by either serial transfer (i.v.) of 10⁶Plasmodium yoelii (Py; American Type Culture Collection) parasitized RBCs (pRBCs) derived from a single donor C57BL/6 mouse, or i.p. injections of either 2.5 × 10⁶ PFUs of LCMV-Arm or 5,000 Tb Antat1.1 parasites (Tb; a generous gift from Kent Hill, University of California, Los Angeles, Los Angeles, CA). In some experiments, Py-infected mice were treated with 500 µg of either α-IFN-γ (clone XMG1.2; BioXcell) or rat IgG1 isotype control or with 400 µg of either mouse chimeric PtS-blocking antibody clone mch1N11 or mouse IgG2a (clone 1.18.4; BioXcell) isotype control, as indicated in the figure legends. Parasitemia was measured using flow cytometry as described (Malleret et al., 2011 (link); Villarino et al., 2016 (link)).